Spin label detection of intermolecular interactions in carbonmonoxy sickle hemoglobin
نویسندگان
چکیده
منابع مشابه
Heme-Spin Label Studies of Hemoglobin
In order to investigate the protein conformation in the vicinity of the heme and the spin states of the heme-iron in dissolved hemoglobins, a nitroxide spin label was covalently attached to one of the propionic acid groups of the porphyrin ring. The optical spectra of the heme-spin labeled ferrihemoglobins were identical to those of native hemoglobins indicating that the spin labeling did not a...
متن کاملSpin-label study of hemoglobin conformations in solution.
There is now a great deal of physical and chemical evidence that horse and human hemoglobins undergo a change of protein conformation on oxygenation.' 9 The molecular mechanism whereby these hemoglobins show cooperative or sigmoidal oxygen binding has remained obscure, however. In order to understand this "heme-heme" interaction, one must obtain structural information on partially oxygenated he...
متن کاملBackbone dynamics of deoxy and carbonmonoxy hemoglobin by NMR/SRLS.
The slowly relaxing local structure (SRLS) approach, developed for NMR spin relaxation analysis in proteins, is applied herein to amide ¹⁵N relaxation in deoxy and carbonmonoxy hemoglobin. Experimental data including ¹⁵N T₁, T₂ and ¹⁵N-{¹H} NOE, acquired at 11.7 and 14.1 T, and 29 and 34 °C, are analyzed. The restricted local motion of the N-H bond is described in terms of the principal value (...
متن کاملDenatured hemoglobin in sickle erythrocytes.
To study the nature of numerous inclusion bodies seen in red cells from patients with sickle cell disease (Hb SS), we have prepared red cell ghosts free of oxyhemoglobin and analyzed them by spectrophotometric and heme extraction methods. The absorption spectrum in the visible region of the ghost suspensions was typical of hemichromes. The spectrum was similar to that of denatured hemoglobin re...
متن کاملSynthesis and membrane interactions of spin-label bifunctional reagents.
The design of chemical reagents that interact with biological membranes requires knowledge of the orientation and location of the reagents with respect to the membrane surface and of the partition coefficients of the reagents between membrane and water. These properties are explored here for a class of molecules having the following properties: (1) they have long, flexible chains which are, in ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1978
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(78)85398-3